A Simple Key For cytometer Unveiled

A Simple Key For cytometer Unveiled

Blog Article

Info-enriched characterization of cell populations. Morphological data from visuals can incorporate to your richness of flow cytometry facts. As an example, the determine exhibits an in any other case traditional apoptosis assay working with Annexin V and PI, including cell imaging to characterize cells in Each and every inhabitants to expose morphologically unique characteristics. These insights could not are actually received from multiplex staining on your own.

As samples are acquired to the Attune CytPix Flow Cytometer, the large-speed brightfield digicam captures and outlets pictures of detected occasions at a amount around 6,000 photographs per 2nd, depending upon the flow rate and image measurement. For larger adaptability, Attune Cytometric Software enables you to adjust the picture seize frequency as wanted and select a specific gate for graphic capture, if required.

(B) Uncooked 264.7 macrophages are sometimes annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The quantity of singlets available for Evaluation will increase after the consumer trains and employs a far more exact design.

Explore new insights into your experimental knowledge by visualizing populations applying combined fluorescence and morphology info. Our application interface wizards empower downsampling, concatenation (merged information) and dimensionality tools which are built in the computer software for an intuitive, streamlined information Evaluation knowledge.

"A picture isn't really pretty truly worth one thousand dots, but it definitely really helps to see images. It is excellent to determine pictures that correlate with staining. As an example, AnnexinV-beneficial cells that happen to be morphologically various than wholesome cells, or CD14+ monocytes which are greater and a lot more textured than CD3+/CD4+/CD14- lymphocytes.

In this article, a seasoned consumer has gated singlets confidently. Soon after assessing the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate incorporates greater than 4% aggregates.

Pair your Attune flow cytometer with an autosampler for improved workflow efficiency, and include robotic integration for max throughput.

Dependable picture high-quality even at substantial throughput Acoustic focusing along with a high-speed digicam Incorporate to graphic these T cells continually at very low or large flow prices. Easily modify concentration and camera configurations to satisfy experimental specifications.

The Attune NxT cytometer is definitely the responsible workhorse instrument your lab can rely on for dependable and reliable performance. The Attune CytPix model features a substantial-pace brightfield camera and AI-pushed graphic analysis software, with recent updates such as device Mastering instruments and an intuitive user interface, enabling Increased details precision, precision, and speedier insights into sample biology.

Users can view event illustrations or photos in real-time to verify sample high quality, optimize protocols, and document the morphology of each and every cell inhabitants discovered for Investigation. Imaging capture also Rewards from acoustic concentrating, which really helps to placement the cells so that a pointy, centered picture is received.

Audio waves empower precise mobile alignment, resulting in exceptional knowledge high-quality and ~10X more quickly sample flow prices.

Application Take note: Automatic impression analysis reduces user-to-user variability in flow cytometry gating approaches

Leverage the strength of Highly developed engineering and innovation to accelerate your discoveries. Have confidence in during the Attune Flow Cytometer family members to provide the dependability, pace, and accuracy that established your get the job done aside.

No wash/no lyse leukocyte analysis with imaging. Cells were obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Qualified UX/UI allows customers to train AI types for expanded purposes. (A) The “Practice Model” feature delivers annotation resources and authentic-time product teaching precision.

At the center of every Attune Flow Cytometer lies our innovative acoustic focusing technological innovation, which utilizes click here audio waves to meticulously align cells in the flow cell. This impressive method surpasses regular hydrodynamic solutions, leading to a lot quicker processing speeds, Remarkable details high-quality, and increased sample throughput.

The Attune Xenith cytometer can be a significant-parameter spectral analyzer, furnishing each spectral unmixing and standard flow capabilities for extensive and flexible cell Evaluation.

The Attune CytPix Flow Cytometer permits end users to visualise samples during acquisition, verify the precision of gating procedures, and guidance final results with reputable documentation for publication uses.

Right here, we display that by gating techniques applying impression-dependent quantitative parameters can distinguish interacting cells from coincident occasions to far more precisely evaluate interacting cells.